1 ap proteintech rabbit anti scd polyclonal antibody  (Proteintech)

Journal: bioRxiv

Article Title: TSPO deficiency reveals a novel role for porphyrins in regulating white adipose tissue lipid metabolism

doi: 10.1101/2025.09.14.676078

Figure Lengend Snippet: (A) Representative Oil Red O staining of primary adipocytes derived from Tspo fl/fl and Tspo -/- mice at Day 4 of diberentiation under adipogenic conditions indicated higher abundance of lipid accumulation in Tspo -/- adipocytes. (B) Graph showing quantification of lipid accumulation by Oil Red O absorbance at Day 0 and Day 4 of diberentiation. Tspo -/- adipocytes showed significantly higher levels of lipid accumulation compared to Tspo fl/fl controls. (C) Quantitative PCR analysis of Tspo mRNA expression during diberentiation (Days 0, 2, and 4) in Tspo fl/fl adipocytes demonstrates robust induction of Tspo transcript levels over the course of adipogenesis. (D) Western blot analysis of TSPO, PPARγ, and HSP90 protein levels at Days 0, 2, and 4 of diberentiation in Tspo fl/fl adipocytes indicated upregulation of TSPO and PPARγ at Day 2 and 4; HSP90 levels remained unchanged with diberentiation. Actin is shown as a loading control. (E) Western blot analysis of PPARγ and FABP4 protein expression in Day 4 adipocytes from two independent replicate samples of Tspo fl/fl and Tspo -/- mice. Tspo -/- cells show reduced PPARγ and FABP4 protein levels relative to controls. Actin serves as a loading control. (F) Relative mRNA expression of adipogenesis-associated genes ( Pparg, Fabp4, Cd36, Lpl, Glut4, Srebp1c, Fasn, Acaca, Hsl, Atgl ) in Tspo fl/fl and Tspo -/- adipocytes at Days 0 and 4. At Day 4, Tspo -/- adipocytes exhibit significant downregulation of Fabp4 , Acaca , Hsl , and Atgl , consistent with impaired adipogenic and lipolytic gene programs (mean ± SEM; *p < 0.05).

Article Snippet: Primary antibody incubations were performed overnight at 4°C using the following antibodies: rabbit anti-TSPO (EPR5384) monoclonal antibody (Abcam, #ab109497), rabbit anti-HSP90 (C45G5) monoclonal antibody (Cell Signaling, #4877), rabbit anti-PPARγ monoclonal antibody (Cell Signaling, #81B8), rabbit anti-FABP4 polyclonal antibody (Cell Signaling, #2120) and mouse monoclonal anti-β-actin antibody (Li-Cor, 926-42212) as a loading control.

Techniques: Staining, Derivative Assay, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Control

Journal: bioRxiv

Article Title: TSPO deficiency reveals a novel role for porphyrins in regulating white adipose tissue lipid metabolism

doi: 10.1101/2025.09.14.676078

Figure Lengend Snippet: (A) Baseline protoporphyrin IX (PPIX) concentrations measured in WAT was not diberent between Tspo fl/fl and Tspo -/- mice under standard chow conditions. (B) Relative mRNA expression of δ-aminolevulinic acid synthase ( Alas ) and ferrochelatase ( Fech ) in WAT under HF diet conditions showed significant downregulation in Tspo -/- mice compared to Tspo fl/fl controls. (C-D) During in vitro adipocyte diberentiation, Tspo -/- primary adipocytes showed significantly elevated expression of Alas and Fech at days 2 and 4 compared to Tspo fl/fl cells, suggesting dysregulation of porphyrin and heme biosynthetic genes in the absence of TSPO. (E) Adipogenic gene expression in Tspo fl/fl primary adipocytes treated with δ-aminolevulinic acid (ALA), protoporphyrin IX (PPIX), or hemin during diberentiation at Day 4. Treatment with ALA and PPIX significantly reduced mRNA levels of the adipogenic markers Pparg and Fabp4 , whereas hemin had no significant ebect. Data are presented as mean ± SEM. Diberent letters denote statistically significant diberences among groups (p < 0.05).

Article Snippet: Primary antibody incubations were performed overnight at 4°C using the following antibodies: rabbit anti-TSPO (EPR5384) monoclonal antibody (Abcam, #ab109497), rabbit anti-HSP90 (C45G5) monoclonal antibody (Cell Signaling, #4877), rabbit anti-PPARγ monoclonal antibody (Cell Signaling, #81B8), rabbit anti-FABP4 polyclonal antibody (Cell Signaling, #2120) and mouse monoclonal anti-β-actin antibody (Li-Cor, 926-42212) as a loading control.

Techniques: Expressing, In Vitro, Gene Expression

Journal: iScience

Article Title: Vitamin D alleviates HFD-induced hepatic fibrosis by inhibiting DNMT1 to affect the TGFβ1/Smad3 pathway

doi: 10.1016/j.isci.2024.111262

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-FABP4 , Cell Signaling Technology , Cat#50699.

Techniques: Recombinant, Methylation, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, Sequencing, Negative Control, Positive Control, Software

Journal: The Journal of reproduction and development

Article Title: FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation.

doi: 10.1262/jrd.2023-015

Figure Lengend Snippet: Fig. 1. Expression levels of FABP4 in sheep endometrium. (a) FABP4 mRNA expression in sheep endometrium on day 4 and day 15. (b, c) FABP4 protein expression in sheep endometrium on day 4 and 15. The optical density was normalized to the density of β-actin in the same lane. (d, e) Rabbit IgG group was used as the negative control for analyzing the localization and expression of FABP4 in sheep endometrium. Data are presented as mean ± standard error with significant differences at P < 0.05 and extremely significant differences at P < 0.01. * indicates P < 0.05, ** indicates P < 0.01, and no sign indicates that the difference is not significant. The technique was repeated thrice.

Article Snippet: SEECs were fixed with 4% paraformaldehyde at room temperature, blocked with BSA for 30 min, and FABP4 rabbit polyclonal antibody (Boster, BM4029, 1:50) and CK18 mouse monoclonal antibody (Abcam, Cambridge, UK, ab668, 1:50) were added overnight at 4°C.

Techniques: Expressing, Negative Control

Journal: The Journal of reproduction and development

Article Title: FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation.

doi: 10.1262/jrd.2023-015

Figure Lengend Snippet: Fig. 2. Expression of FABP4 in SEECs. (a) Isolation and purification of SEECs (250 ×). When the cells reached the sixth passage, they became larger and rounder and began to senesce. (b) Immunofluorescence identification of SEECs (CK18) (100 ×). (c) Localization of FABP4 in SEECs (Yellow arrows are FABP4 in the nuclei) (25 ×).

Article Snippet: SEECs were fixed with 4% paraformaldehyde at room temperature, blocked with BSA for 30 min, and FABP4 rabbit polyclonal antibody (Boster, BM4029, 1:50) and CK18 mouse monoclonal antibody (Abcam, Cambridge, UK, ab668, 1:50) were added overnight at 4°C.

Techniques: Expressing, Isolation, Purification, Immunofluorescence

Journal: The Journal of reproduction and development

Article Title: FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation.

doi: 10.1262/jrd.2023-015

Figure Lengend Snippet: Fig. 3. Hormone treatment of SEECs and detection of changes in FABP4 expression. (a) Protein expression levels of PGR and ER after combined hormone treatment. (b) The mRNA expression levels of ISG15, HOXA10, CXCL10, and RSAD2 after hormone treatment. (c) Levels of the prostaglandin secreted from the SEECs after hormone treatment.(d) Expression levels of FABP4 after hormone treatment. All data are presented as mean ± standard error. Differences were considered significant at P < 0.05 and extremely significant at P < 0.01.

Article Snippet: SEECs were fixed with 4% paraformaldehyde at room temperature, blocked with BSA for 30 min, and FABP4 rabbit polyclonal antibody (Boster, BM4029, 1:50) and CK18 mouse monoclonal antibody (Abcam, Cambridge, UK, ab668, 1:50) were added overnight at 4°C.

Techniques: Expressing

Journal: The Journal of reproduction and development

Article Title: FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation.

doi: 10.1262/jrd.2023-015

Figure Lengend Snippet: Fig. 4. FABP4 inhibition impedes SEEC function. (a, b) Mobility of SEECs at 0, 24, 48, 72 and 96 h was measured using a scratch test. Migratory capacity was calculated as a percentage of healing area relative to time 0. (c) CCK-8 viable cell counts quantified the proliferative capacity of SEECs treated with hormone and FABP4 inhibitor BMS309403. (d–g) Expression levels of EMT after hormone and inhibitor treatment (E-cadherin, N-cadherin, Vim, and β-catenin). (h, i) Changes in endoplasmic reticulum stress-related protein CHOP and GRP78 were measured. (j, k) Changes in autophagy- related proteins p-mTOR, LC3B II/I, and P62. Data are expressed as mean ± standard error. Differences were considered significant at P < 0.05 and extremely significant at P < 0.01.

Article Snippet: SEECs were fixed with 4% paraformaldehyde at room temperature, blocked with BSA for 30 min, and FABP4 rabbit polyclonal antibody (Boster, BM4029, 1:50) and CK18 mouse monoclonal antibody (Abcam, Cambridge, UK, ab668, 1:50) were added overnight at 4°C.

Techniques: Inhibition, CCK-8 Assay, Expressing

Journal: The Journal of reproduction and development

Article Title: FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation.

doi: 10.1262/jrd.2023-015

Figure Lengend Snippet: Fig. 5. TG and 3-MA treatment partially restores SEEC function after BMS30940 suppression of FABP4. (a, b) Expression levels of key proteins in the endoplasmic reticulum stress signaling pathway after combined treatment with hormones, BMS309403, and TG. (c, d) Expression levels of key proteins in autophagy and apoptosis signaling pathways after combined treatment with hormones, BMS309403, and 3-MA. (e) Secreted prostaglandin levels from SEECs after combined treatment with hormones, BMS309403, and TG or 3-MA. Data are expressed as mean ± standard error. Differences were considered significant at P < 0.05 and extremely significant at P < 0.01.

Article Snippet: SEECs were fixed with 4% paraformaldehyde at room temperature, blocked with BSA for 30 min, and FABP4 rabbit polyclonal antibody (Boster, BM4029, 1:50) and CK18 mouse monoclonal antibody (Abcam, Cambridge, UK, ab668, 1:50) were added overnight at 4°C.

Techniques: Expressing, Protein-Protein interactions

Journal: iScience

Article Title: TET2 is recruited by CREB to promote Cebpb , Cebpa , and Pparg transcription by facilitating hydroxymethylation during adipocyte differentiation

doi: 10.1016/j.isci.2023.108312

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-FABP4 , Proteintech , Cat#12802-1-AP; RRID: AB_2102442.

Techniques: Virus, Plasmid Preparation, Recombinant, Transfection, Lysis, CCK-8 Assay, BIA-KA, Cholesterol Assay, CRISPR, Software, Magnetic Beads